Subcellular Localization of Glycosphingolipid by Immuno-Electronmicroscopy after Freeze-Substitution
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چکیده
منابع مشابه
Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution
Forssman antigen, a neutral glycosphingolipid carrying five monosaccharides, was localized in epithelial MDCK cells by the immunogold technique. Labeling with a well defined mAb and protein A-gold after freeze-substitution and low temperature embedding in Lowicryl HM20 of aldehyde-fixed and cryoprotected cells, resulted in high levels of specific labeling and excellent retention of cellular ult...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 1992
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.4.299